Ghent University Academic Bibliography


Project: Development of novel expression technology for eukaryotic membrane proteins: application to G-protein coupled receptors

project duration
01-OCT-09 – 30-SEP-11
(1) Integration of several engineering steps for the optimalisation of GPCR overexpression in engineered east strains with increased ER-membrane capacity and use of an existing (T4 lysozyme insertion) and a new (nanobody complexation by coƫxpression) method for GPCR-stabilisation and crystallisation (2) Production, purification and crystallization of the GPCR membrane proteins