Project: Analysis of the alternative splicing pattern of the human interleukin 5 alpha-receptor using reporte systems.
01-JUL-97 – 31-DEC-99
In this program we plan to determine the alternative splicing pattern of transcripts of the human interleukin-5- alpha-receptor gene. Via this mechanism, either a secreted or a membrane-anchored receptor variant can be generated. We will use minigene-constructs in which coding regions for interleukin-6 or luciferase are coupled to the extracellular or cytoplasmic regions of IL5Ra respectively. This will allows efficient analysis of the isoform expression pattern.