Project: Optimalization of purification techniques for heterologous proteins expressed in Escherichia coli.
- project duration
- 31-DEC-99 – 31-DEC-99
- The project includes the following aspects : - synthesis efficiency of a cloned protein - study of the formation of inclusion bodies and growth conditions, role of chaperonne proteins - purification of native proteins from inclusion bodies controlled denaturation and renaturation techniques. The model system used includes cloned murine cytokines expressed in E. coli after purification the proteins will be checked for immunological functions in test animal models.