Project: Genetic aspects of the biosynthesis of the Lactococcus lactis subsp. lactis. Lantibiotic Nisin.
- project duration
- 01-JAN-00 – 31-DEC-99
- Elucidation of the molecular nisin biosynthesis pathway in Lactococcus lactis subsp. lactis and its genetic regulation by : 1) isolation and genetic characterization of genomic and plasmid-DNA 2) conjugation of the sucrose and nisin genes 3) transposon mutagenesis of the nisin biosynthesis genes 4) electroporation and transformation of nisin producing and non-nisin producing strains 5) hybridization-experiments to characterize the genetic information and organization of the nisin genes.