em. prof. dr. Anna Depicker
- ORCID iD
- 0000-0003-0105-7407
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Using GlycoDelete to produce proteins lacking plant-specific N-glycan modification in seeds (vol 33, pg 1135, 2019)
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- Journal Article
- A1
- open access
Seed-produced anti-globulin VHH-Fc antibodies retrieve globulin precursors in the insoluble fraction and modulate the Arabidopsis thaliana seed subcellular morphology
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- Journal Article
- A1
- open access
Evaluating single-domain antibodies as carriers for targeted vaccine delivery to the small intestinal epithelium
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- Journal Article
- A1
- open access
Simplified monomeric VHH-Fc antibodies provide new opportunities for passive immunization
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- Journal Article
- A1
- open access
Russell-like bodies in plant seeds share common features with prolamin bodies and occur upon recombinant protein production
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- Journal Article
- A1
- open access
Transformation strategies for stable expression of complex hetero-multimeric proteins like secretory immunoglobulin A in plants
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- Journal Article
- A1
- open access
Yeast-secreted, dried and food-admixed monomeric IgA prevents gastrointestinal infection in a piglet model
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A two-amino acid mutation in murine IgA enables downstream processing and purification on staphylococcal superantigen-like protein 7.
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Plant seed production of biopharmaceuticals : bridging red and green biotechnology
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- Journal Article
- A1
- open access
In planta expression of nanobody-based designer chicken antibodies targeting Campylobacter
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High accumulation in tobacco seeds of hemagglutinin antigen from avian (H5N1) influenza
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The case for plant-made veterinary immunotherapeutics
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- Journal Article
- A1
- open access
Biomanufacturing of protective antibodies and other therapeutics in edible plant tissues for oral applications
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Recombinant IgA production for mucosal passive immunization, advancing beyond the hurdles
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- Conference Paper
- C3
- open access
Evaluating recombinant secretory IgA stability upon a feed challenge experiment
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- Conference Paper
- C3
- open access
IgA-based oral passive immunisation against enterotoxigenic Escherichia coli
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Plant expression systems for early stage discovery and development of lead therapeutic antibodies
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Using GlycoDelete to produce proteins lacking plant-specific N-glycan modification in seeds
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Tobacco seeds as efficient production platform for a biologically active anti-HBsAg monoclonal antibody
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- Conference Paper
- C3
- open access
Advantages of plant expression systems for the rational design of oral therapeutics
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Comparison of VHH-Fc antibody production in Arabidopsis thaliana, Nicotiana benthamiana and Pichia pastoris
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- Conference Paper
- C3
- open access
Oral passive immunization with seeds producing heavy-chain-only IgAs prevents enterotoxigenic Escherichia coli infection in piglets
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Chlamydia psittaci vaccination in SPF chickens using the recombinant major outer membrane protein
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Chlamydia psittaci vaccination in SPF chickens using the recombinant major outer membrane protein
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- Journal Article
- A1
- open access
Trafficking of endoplasmic reticulum-retained recombinant proteins is unpredictable in Arabidopsis thaliana
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Single-domain antibodies targeting neuraminidase protect against an H5N1 influenza virus challenge
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Nanobody-based products as research and diagnostic tools
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- Journal Article
- A1
- open access
Boosting in planta production of antigens derived from the porcine reproductive and respiratory syndrome virus (PRRSV) and subsequent evaluation of their immunogenicity
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Detection and investigation of transitive gene silencing in plants
(2014) Plant epigenetics and epigenomics : methods and protocols. In Methods in Molecular Biology 1112. p.219-241 -
Generation of VHH antibodies against the Arabidopsis thaliana seed storage proteins
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The efficiency of Arabidopsis thaliana floral dip transformation is determined not only by the Agrobacterium strain used but also by the physiology and the ecotype of the dipped plant
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Fundamentals of plant biotechnology
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Role of plant expression systems in antibody production for passive immunization
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Epigenetic switches of tobacco transgenes associate with transient redistribution of histone marks in callus culture
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T-DNA transfer and T-DNA integration efficiencies upon Arabidopsis thaliana root explant cocultivation and floral dip transformation
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Fusion of an Fc chain to a VHH boosts the accumulation levels in Arabidopsis seeds
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Transitive RNA silencing signals induce cytosine methylation of a transgenic but not an endogenous target
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- Conference Paper
- C3
- open access
Passive mucosal immunisation with novel simplified IgA antibodies produced in seeds prevents enterotoxigenic Escherichia coli (ETEC) infection in weaned piglets
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Orally fed seeds producing designer IgAs protect weaned piglets against enterotoxigenic Escherichia coli infection
(2013) PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 110(29). p.11809-11814 -
Site-specific T-DNA integration in Arabidopsis thaliana mediated by the combined action of CRE recombinase and φC31 integrase
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Transgene expression in plants, control of
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Recombinant antibody production in Arabidopsis seeds triggers an unfolded protein response
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Transgene expression in plants, control of
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Production of camel-like antibodies in plants
(2012) Single domain antibodies : methods and protocols. In Methods in Molecular Biology 911. p.305-324 -
High frequency of single-copy T-DNA transformants produced after floral dip in CRE-expressing Arabidopsis plants
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Recombinant plant made antibodies against fimbriae of enterotoxigenic Escherichia coli inhibit in vitro binding to villous enterocytes
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- Conference Paper
- C3
- open access
Seed produced anti ETEC antibodies for oral passive immunisation of piglets against post weaning diarrhoea
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Opportunities and challenges for molecular farming in Flanders
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Characterization of the single-chain Fv-Fc antibody MBP10 produced in Arabidopsis alg3 mutant seeds
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Non-food/feed seeds as biofactories for the high-yield production of recombinant pharmaceuticals
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Paramutation of tobacco transgenes by small RNA-mediated transcriptional gene silencing
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Expression of antibody fragments with a controlled N-glycosylation pattern and induction of endoplasmic reticulum-derived vesicles in seeds of Arabidopsis
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Production of monoclonal antibodies with a controlled N-glycosylation pattern in seeds of Arabidopsis thaliana
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Introns reduce transitivity proportionally to their length, suggesting that silencing spreads along the pre-mRNA
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Gene silencing induced by hairpin or inverted repeated sense transgenes varies among promoters and cell types
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Evaluation of seven promoters to achieve germline directed Cre-lox recombination in Arabidopsis thaliana
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The T-DNA integration pattern in Arabidopsis transformants is highly determined by the transformed target cell
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High frequency of single-copy T-DNA transformants produced by floral dip in CRE-expressing Arabidopsis plants
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Production of antibody fragments in Arabidopsis seeds
(2009) Recombinant proteins from plants : methods and protocols. In Methods in Molecular Biology 483. p.89-101 -
Cell culture-induced gradual and frequent epigenetic reprogramming of invertedly repeated tobacco transgene epialleles
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Agrobacterium tumefaciens-mediated transformation: patterns of T-DNA integration into the host genome
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Base excision repair and its role in maintaining genome stability
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Trans-generation inheritance of methylation patterns in a tobacco transgene following a post-transcriptional silencing event
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Transgene locus structure and expression
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Stability of the T-DNA flanking regions in transgenic Arabidopsis thaliana plants under influence of abiotic stress and cultivation practices
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- Journal Article
- A1
- open access
Development and application of novel constructs to score C:G-to-T:A transitions and homologous recombination in Arabidopsis
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Evaluation of CRE-mediated excision approaches in Arabidopsis thaliana
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The stability of T-DNA integration sites in the genome of Arabidopsis thaliana
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- Journal Article
- A1
- open access
Recombinational cloning with plant gateway vectors
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The influence of matrix attachment regions on transgene expression in Arabidopsis thaliana wild type and gene silencing mutants
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Aberrant localization and underglycosylation of highly accumulating single-chain Fv-Fc antibodies in transgenic Arabidopsis seeds
(2007) PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 104(4). p.1430-1435 -
- Journal Article
- A1
- open access
Generation of single-copy T-DNA transformants in Arabidopsis by the CRE/loxP recombination-mediated resolution system
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Down-regulation of endogenes mediated by a transitive silencing signal
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- Journal Article
- A1
- open access
The trans-silencing capacity of invertedly repeated transgenes depends on their epigenetic state in tobacco
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Introduction of silencing-inducing transgenes does not affect expression of known transcripts
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Insights into recognition of the T-DNA border repeats as termination sites for T-strand synthesis by Agrobacterium tumefaciens
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- Journal Article
- A1
- open access
The frequency and efficiency of endogene suppression by transitive silencing signals is influenced by the length of sequence homology
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Sequence stability of the T-DNA - plant junctions in tissue culture in Arabidopsis transgenic lines
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Transgene silencing
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Transitive and systemic RNA silencing: both involving an RNA amplification mechanism?
(2005) Small RNAs : analysis and regulatory functions. In Nucleic Acids and Molecular Biology 17. p.119-139 -
Gene expression and level of expression
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A technology platform for the fast production of monoclonal recombinant antibodies against plant proteins and peptides
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Single-copy T-DNAs integrated at different positions in the Arabidopsis genome display uniform and comparable β-glucuronidase accumulation levels
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Stable high-level transgene expression in Arabidopsis thaliana using gene silencing mutants and matrix attachment regions
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Spreading of post-transcriptional gene silencing along the target gene promotes systemic silencing
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- Journal Article
- A1
- open access
The preferred route for the degradation of silencing target RNAs in transgenic plants depends on pre-established silencing conditions
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Exceptionally high heterologous protein levels in transgenic dicotyledonous seeds using Phaseolus vulgaris regulatory sequences
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T-DNA integration in Arabidopsis chromosomes: presence and origin of filler DNA sequences
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RNA target sequences promote spreading of RNA silencing
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Qualitative and event-specific PCR real-time detection methods for StarLink maize
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Epigenetic switch from posttranscriptional to transcriptional silencing is correlated with promoter hypermethylation
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Genetic and epigenetic aspects of somaclonal variation: flower colour bud sports in azalea, a case study
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GATEWAY™ vectors for Agrobacterium-mediated plant transformation
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Transient expression systems for the production of recombinant antibodies in plants
(2002) Biotechnological and Genetic Engineering. In Recent Progress in Medicinal Plants 4. p.295-306 -
An active role for endogenous β-1,3-glucanase genes in transgene-mediated co-suppression in tobacco
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Identification and characterization of T-DNA inserts by T-DNA fingerprinting
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Boosting heterologous protein production in transgenic dicotyledonous seeds using Phaseolus vulgaris regulatory sequences
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Expression of antibodies and Fab fragments in transgenic potato plants: a case study for bulk production in crop plants
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Disruption of their palindromic arrangement leads to selective loss of DNA methylation in inversely repeated gus transgenes in Arabidopsis
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Silencing of invertedly repeated transgenes in Arabidopsis thaliana.