Academic Bibliography
https://biblio.ugent.be/
Ghent University Academic Bibliography2000-01-01T00:00+00:001monthlyFluorescence, Raman and Cryo-SEM microscopy to investigate plant cell wall polysaccharides in food waste streams
https://biblio.ugent.be/publication/01HSPM0D3HKC12FBB5Q0M0N56V
Verwee, EllenVan de Walle, DavySkirtach, AndreVan Damme, ElsDewettinck, Koen2023application/pdfhttps://biblio.ugent.be/publication/01HSPM0D3HKC12FBB5Q0M0N56Vhttp://hdl.handle.net/1854/LU-01HSPM0D3HKC12FBB5Q0M0N56Vhttps://biblio.ugent.be/publication/01HSPM0D3HKC12FBB5Q0M0N56V/file/01HSPMKFVBX8DSPQCCRRM3YZT9engNo license (in copyright)info:eu-repo/semantics/closedAccessXVI Plant Cell Wall Meeting, AbstractsBiology and Life SciencesFluorescence, Raman and Cryo-SEM microscopy to investigate plant cell wall polysaccharides in food waste streamsconferenceinfo:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionMicroscopy techniques as tools to understand the microstructure of food processing by-products
https://biblio.ugent.be/publication/01HSPK5EG5EPMSCHP3B1QE0APY
Verwee, EllenVanleenhove, BaptisteVan den Wouwer, BenRaes, KatleenVan de Walle, DavyDewettinck, KoenSkirtach, AndreVan Damme, Els2023application/pdfhttps://biblio.ugent.be/publication/01HSPK5EG5EPMSCHP3B1QE0APYhttp://hdl.handle.net/1854/LU-01HSPK5EG5EPMSCHP3B1QE0APYhttps://biblio.ugent.be/publication/01HSPK5EG5EPMSCHP3B1QE0APY/file/01HSPKEKZKDKYJ9PJHCPDB4SZWengNo license (in copyright)info:eu-repo/semantics/closedAccessFood Chemistry, 3rd Conference, AbstractsAgriculture and Food SciencesMicroscopy techniques as tools to understand the microstructure of food processing by-productsconferenceinfo:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionVisualisation of microalgal lipid bodies through electron microscopy
https://biblio.ugent.be/publication/01HPGT7P6TNY9V5WC4NS7YGRQH
Verwee, EllenVan de Walle, DavyDe Bruyne, MichielMienis, EstherSekulić, MirnaChaerle, PeterVyverman, WimFoubert, ImogenDewettinck, Koen2024In this study, transmission electron microscopy (TEM) and cryo-scanning electron microscopy (cryo-SEM) were evaluated for their ability to detect lipid bodies in microalgae. To do so, Phaeodactylum tricornutum and Nannochloropsis oculata cells were harvested in both the mid-exponential and early stationary growth phase. Two different cryo-SEM cutting methods were compared: cryo-planing and freeze-fracturing. The results showed that, despite the longer preparation time, TEM visualisation preceded by cryo-immobilisation allows a clear detection of lipid bodies and is preferable to cryo-SEM. Using freeze-fracturing, lipid bodies were rarely detected. This was only feasible if crystalline layers in the internal structure, most likely related to sterol esters or di-saturated triacylglycerols, were revealed. Furthermore, lipid bodies could not be detected using cryo-planing. Cryo-SEM is also not the preferred technique to recognise other organelles besides lipid bodies, yet it did reveal chloroplasts in both species and filament-containing organelles in cryo-planed Nannochloropsis oculata samples.
application/pdfhttps://biblio.ugent.be/publication/01HPGT7P6TNY9V5WC4NS7YGRQHhttp://hdl.handle.net/1854/LU-01HPGT7P6TNY9V5WC4NS7YGRQHhttp://doi.org/10.1111/jmi.13259https://biblio.ugent.be/publication/01HPGT7P6TNY9V5WC4NS7YGRQH/file/01HPGTE45QQ75DN2HR7ADSMCMMengNo license (in copyright)info:eu-repo/semantics/closedAccessJOURNAL OF MICROSCOPYISSN: 0022-2720ISSN: 1365-2818Biology and Life Sciencescryo-planingelectron microscopyfreeze-fracturinglipid bodiesNannochloropsis oculataPhaeodactylum tricornutumNANNOCHLOROPSIS-OCULATANITROGEN STARVATIONTRANSMISSIONDROPLETSGROWTHBIOMASSVisualisation of microalgal lipid bodies through electron microscopyjournalArticleinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion