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Ghent University Academic Bibliography2000-01-01T00:00+00:001dailyExperimental accumulation and depuration kinetics and natural occurrence of microcystin-LR in basil (Ocimum basilicum L.)
https://biblio.ugent.be/publication/01HRHFC0FJED8N5BGAS656N3XZ
Fossi, Maria CristinaVan Hassel, WannesAbdallah, Mohamed FathiGuzman Velasquez, Maria GraciaMiles, Christopher O.Samdal, Ingunn A.Masquelier, JulienRajkovic, Andreja2024info:eu-repo/date/embargoEnd/2024-09-20Microcystin-LR (MC-LR) is a hepatotoxic metabolite that naturally occurs during some cyanobacterial blooms in eutrophic waterbodies, and irrigation of edible plants with MC-LR-contaminated water causes bioaccumulation of the toxin. However, sufficient information about accumulation and depuration mechanics in hydroculture-grown herb plants is still lacking. This work aimed at 1) investigating bioaccumulation and depuration of MC-LR in basil, 2) verifying the possible MC-LR detoxification mechanisms in the plant, and 3) detecting the natural occurrence of MC-LR in basil (n = 50) collected from the Belgian market. Basil plants grown in a hydroculture were exposed to MC-LR (5, 20, and 50 μg L−1) spiked in a Hoagland solution for seven days. MC-LR depuration was also studied by transferring the plants to a non-contaminated Hoagland solution after exposure to MC-LR for another seven days. MC-LR concentrations in Hoagland solution, basil leaves, and roots were quantified using a validated UHPLC–MS/MS method. In addition, ELISA and LC–HRMS (only basil leaves) were used for confirmation. The results showed an increase in the accumulated levels of MC-LR at higher exposure doses, with higher MC-LR levels in roots than in leaves for all the treatment conditions. For MC-LR depuration, significant reductions were observed in all the treatment conditions for roots only. No MC-LR conjugates, potentially related to metabolism, were detected by LC–HRMS. Finally, MC-LR was detected in one store-bought basil sample, representing the first occurrence of cyanotoxins in an edible crop from Belgium.application/pdfhttps://biblio.ugent.be/publication/01HRHFC0FJED8N5BGAS656N3XZhttp://hdl.handle.net/1854/LU-01HRHFC0FJED8N5BGAS656N3XZhttp://doi.org/10.1016/j.envpol.2024.123715https://biblio.ugent.be/publication/01HRHFC0FJED8N5BGAS656N3XZ/file/01HRHFGBAH2CZMDTJYK8Q63SHEengNo license (in copyright)info:eu-repo/semantics/embargoedAccessENVIRONMENTAL POLLUTIONISSN: 0269-7491ISSN: 1873-6424Agriculture and Food SciencesEarth and Environmental SciencesChemistryMicrocystinsMC-LRbasilUHPLC-MS/MSaccumulationdepurationfood safetyExperimental accumulation and depuration kinetics and natural occurrence of microcystin-LR in basil (Ocimum basilicum L.)journalArticleinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion